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dcfda cellular reactive oxygen species detection assay kit  (Abcam)


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    Abcam dcfda cellular reactive oxygen species detection assay kit
    Dcfda Cellular Reactive Oxygen Species Detection Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dcfda+cellular+reactive+oxygen+species+detection+assay+kit/DCFDA+%2F+H2DCFDA+-+Cellular+ROS+Assay+Kit+300+tests/us11692008-852-0-8
    Average 99 stars, based on 1400 article reviews
    dcfda cellular reactive oxygen species detection assay kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Immune checkpoint inhibitor combinations
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    Article Title: Chemotherapeutic combinations of cationic antimicrobial peptides and chemotherapeutics
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    Detection Assay:

    Article Title: Immune checkpoint inhibitor combinations
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    Article Title: Chemotherapeutic combinations of cationic antimicrobial peptides and chemotherapeutics
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    Article Title: Caveolae-mediated effects of TNF-α on human skeletal muscle cells.
    Article Snippet: Chronic diseases are characterized by the production of pro-inflammatory cytokines such than TNF-α and are frequently correlated with muscle wasting conditions.. Among the pleiotropic effects of TNF-α within the cell, its binding to TNFR1 receptor has been shown to activate sphingomyelinases leading to the production of ceramides.. Sphingomyelinases and TNF receptor have been localized within caveolae which are specialized RAFT enriched in cholesterol and sphingolipids.

    Article Title: Astaxanthin Ameliorates Ischemic-Hypoxic-Induced Neurotrophin Receptor p75 Upregulation in the Endothelial Cells of Neonatal Mouse Brains
    Article Snippet: For this study, the crystal form of AXT (LemnaRed ® Crystal, provided by Lemnaceae Fermentation Inc., Tainan, Taiwan) was extracted and purified from engineered E. coli biomass, which was propagated under optimized aerobic conditions [ , ]. .. Assays used include the DCFDA Cellular Reactive Oxygen Species Detection Assay Kit (Abcam, Burlingame, CA, USA), and the HyECL Western Chemiluminescent Kit (HyECL Biotechnology Inc., Taipei, Taiwan). .. For detecting and quantifying apoptosis at the cellular level, the study used the In Situ Cell Death Detection Fluorescein Kit from Roche Diagnostics (Rotkreuz, Switzerland).

    Article Title: Method for treating damage induced by sleep deprivation
    Article Snippet: .. Any appropriate method can be used, however one way to measure the levels of reactive oxygen species uses, for example, a DCFDA-Cellular reactive oxygen species detection assay kit (ab113851, Abcam; Cambridge, Mass.). .. Any appropriate method can be used, however one way to measure the levels of reactive oxygen species uses, for example, a DCFDA-Cellular reactive oxygen species detection assay kit (ab113851, Abcam; Cambridge, Mass.).

    Article Title: Potential anti-cancer and anti-Candida activity of Zn-derived foams.
    Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the ethical guidelines, outlined in our author and reviewer resource centre, still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

    Incubation:

    Article Title: Immune checkpoint inhibitor combinations
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    Article Title: Chemotherapeutic combinations of cationic antimicrobial peptides and chemotherapeutics
    Article Snippet: Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany). .. Fluorescence Measurement of Reactive Oxygen Species (ROS) A DCFDA cellular reactive oxygen species detection assay kit was purchased from Abcam®, and A375 cells seeded in a 96-well Costar black clear bottom plate with 20,000 cells per well incubated in 37° C. 16 hours prior to DCFDA assay. ..

    other:

    Article Title: Preparation and uses of reactive oxygen species scavenger derivatives
    Article Snippet: Calculate the EC50 value using formula equation 201: y=(A+((B−A)/(1+((x/C){circumflex over ( )}D)))) from the XLFit (Excel add-in software), where A: Average mean of vehicle samples B: Average mean of cells treated with 100 nM AcD C: EC50 D: HillSlope Z′ factor was used to describe the screening assay performance.

    Article Title: Tanycytes Regulate Lipid Homeostasis by Sensing Free Fatty Acids and Signaling to Key Hypothalamic Neuronal Populations via FGF21 Secretion.
    Article Snippet: After 30min of DCFDA incubation, cells were washed and incubated with NAC and/or palmitate before performing Time-lapse analysis using a confocal microscope (inverted Zeiss LSM 710 confocal microscope) or quantitative analysis using a Fluorescencemicroplate reader (Sinergy MX, Biotech) at Excitation 495nmand Emission 529nmwavelengths.

    Western Blot:

    Article Title: Astaxanthin Ameliorates Ischemic-Hypoxic-Induced Neurotrophin Receptor p75 Upregulation in the Endothelial Cells of Neonatal Mouse Brains
    Article Snippet: For this study, the crystal form of AXT (LemnaRed ® Crystal, provided by Lemnaceae Fermentation Inc., Tainan, Taiwan) was extracted and purified from engineered E. coli biomass, which was propagated under optimized aerobic conditions [ , ]. .. Assays used include the DCFDA Cellular Reactive Oxygen Species Detection Assay Kit (Abcam, Burlingame, CA, USA), and the HyECL Western Chemiluminescent Kit (HyECL Biotechnology Inc., Taipei, Taiwan). .. For detecting and quantifying apoptosis at the cellular level, the study used the In Situ Cell Death Detection Fluorescein Kit from Roche Diagnostics (Rotkreuz, Switzerland).



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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a <t>DCFDA/H2DCFDA-Cellular</t> Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.
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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a <t>DCFDA/H2DCFDA-Cellular</t> Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.
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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a <t>DCFDA/H2DCFDA-Cellular</t> Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.
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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a <t>DCFDA/H2DCFDA-Cellular</t> Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.
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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a <t>DCFDA/H2DCFDA-Cellular</t> Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.
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    Nicotine induces and augments pro-inflammatory cytokines and <t>ROS</t> in human AoSMC and murine RAW264.7 cells. (A) Nicotine increases expression levels of inflammatory genes (Ccl2, Il6 and Tnf) in murine RAW264.7 cells. (B) Nicotine dose-dependently augments inflammatory gene (Ccl2, Tnf) responses to recombinant IL-6 treatment (20 ng/mL) in RAW264.7 cells. (C) Nicotine dose-dependently increases total ROS in RAW264.7 cells <t>(DCFDA</t> Assay, Ex/Em = 485/535 nm), a process reversed by TEMPOL. (D) Nicotine dose-dependently increases expression levels of inflammatory genes (CCL2, IL6 and IL8) in human AoSMCs. (E) Nicotine dose-dependently augments inflammatory gene (CCL2, IL6 and IL8) responses to recombinant IL-6 treatment (20 ng/mL) in human AoSMCs. (F) Nicotine dose-dependently increases ROS response to IL6 (20 ng/mL) in human AoSMCs (Abcam/Rhodamine, Ex/Em = 550/620 nm). (G) Corresponding pictures to (F) of human AoSMCs stained for ROS under green channel using ROS Detection Assay (Abcam/Rhodamine). Gene expression data are qRT–PCR presented as fold change vs. control. §P < 0.05 vs. control; vs. §§ control and IL6; † vs. control, IL6, and IL6 + nicotine 10 nM (A, B, D, and E). *P < 0.05 vs. control; #P < 0.05 vs. other group (C). *P < 0.05 or **P < 0.01 vs. IL6, #P < 0.05 vs. other group (F). All two-tailed Student’s t-test. Data are mean ± SEM (n = 4–6/treatment group).
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    Nicotine induces and augments pro-inflammatory cytokines and <t>ROS</t> in human AoSMC and murine RAW264.7 cells. (A) Nicotine increases expression levels of inflammatory genes (Ccl2, Il6 and Tnf) in murine RAW264.7 cells. (B) Nicotine dose-dependently augments inflammatory gene (Ccl2, Tnf) responses to recombinant IL-6 treatment (20 ng/mL) in RAW264.7 cells. (C) Nicotine dose-dependently increases total ROS in RAW264.7 cells <t>(DCFDA</t> Assay, Ex/Em = 485/535 nm), a process reversed by TEMPOL. (D) Nicotine dose-dependently increases expression levels of inflammatory genes (CCL2, IL6 and IL8) in human AoSMCs. (E) Nicotine dose-dependently augments inflammatory gene (CCL2, IL6 and IL8) responses to recombinant IL-6 treatment (20 ng/mL) in human AoSMCs. (F) Nicotine dose-dependently increases ROS response to IL6 (20 ng/mL) in human AoSMCs (Abcam/Rhodamine, Ex/Em = 550/620 nm). (G) Corresponding pictures to (F) of human AoSMCs stained for ROS under green channel using ROS Detection Assay (Abcam/Rhodamine). Gene expression data are qRT–PCR presented as fold change vs. control. §P < 0.05 vs. control; vs. §§ control and IL6; † vs. control, IL6, and IL6 + nicotine 10 nM (A, B, D, and E). *P < 0.05 vs. control; #P < 0.05 vs. other group (C). *P < 0.05 or **P < 0.01 vs. IL6, #P < 0.05 vs. other group (F). All two-tailed Student’s t-test. Data are mean ± SEM (n = 4–6/treatment group).
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    The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a DCFDA/H2DCFDA-Cellular Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.

    Journal: Cell Reports Methods

    Article Title: Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids

    doi: 10.1016/j.crmeth.2024.100778

    Figure Lengend Snippet: The characterization of alcohol dehydrogenases in hALO-ALD models (A) Relative mRNA levels of ADH1B and ALDH1B1 in the control-treated and ethanol-treated hALOs were determined. The relative expression of each gene was normalized against that of 18S rRNA. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (B) The expression of ADH1B and ALDH1B1 was evaluated in the control-treated and ethanol-treated hALOs. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (C) The expression of ADH1B and ALDH1B1 was evaluated in liver tissues from control individuals and three different patients with ALD. The histogram shows the difference in the percentages of ADH1B and ALDH1B1 in each field between control individuals and patients with ALD. The hash (#) represents a donor. There were 10 fields in each group. Scale bar, 50 μm. The results are presented as the mean ± SEM. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05 and ∗∗ p < 0.01. (D) The levels of cellular ROS in the control-treated and ethanol-treated hALOs were determined by a DCFDA/H2DCFDA-Cellular Reactive Oxygen Species Detection Assay Kit. The relative fluorescence intensity of ROS was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. (E) Cell viability in the control-treated and ethanol-treated hALOs was determined. The fluorescence intensity was determined using ImageJ software. Significant differences were determined by unpaired two-tailed paired t tests. Scale bar, 50 μm. The results are presented as the mean ± SEM. n = 3. The asterisk (∗) represents statistical significance compared with the control: ∗ p < 0.05. ADH1B, alcohol dehydrogenase 1B; ALDH1B1, aldehyde dehydrogenase 1 family member B1.

    Article Snippet: DCFDA/H2DCFDA-Cellular Reactive Oxygen Species Detection Assay Kit , Abcam , Cat# ab113851.

    Techniques: Expressing, Detection Assay, Fluorescence, Software, Two Tailed Test

    Journal: Cell Reports Methods

    Article Title: Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids

    doi: 10.1016/j.crmeth.2024.100778

    Figure Lengend Snippet:

    Article Snippet: DCFDA/H2DCFDA-Cellular Reactive Oxygen Species Detection Assay Kit , Abcam , Cat# ab113851.

    Techniques: Virus, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Detection Assay, Quantitative RT-PCR, Software

    Nicotine induces and augments pro-inflammatory cytokines and ROS in human AoSMC and murine RAW264.7 cells. (A) Nicotine increases expression levels of inflammatory genes (Ccl2, Il6 and Tnf) in murine RAW264.7 cells. (B) Nicotine dose-dependently augments inflammatory gene (Ccl2, Tnf) responses to recombinant IL-6 treatment (20 ng/mL) in RAW264.7 cells. (C) Nicotine dose-dependently increases total ROS in RAW264.7 cells (DCFDA Assay, Ex/Em = 485/535 nm), a process reversed by TEMPOL. (D) Nicotine dose-dependently increases expression levels of inflammatory genes (CCL2, IL6 and IL8) in human AoSMCs. (E) Nicotine dose-dependently augments inflammatory gene (CCL2, IL6 and IL8) responses to recombinant IL-6 treatment (20 ng/mL) in human AoSMCs. (F) Nicotine dose-dependently increases ROS response to IL6 (20 ng/mL) in human AoSMCs (Abcam/Rhodamine, Ex/Em = 550/620 nm). (G) Corresponding pictures to (F) of human AoSMCs stained for ROS under green channel using ROS Detection Assay (Abcam/Rhodamine). Gene expression data are qRT–PCR presented as fold change vs. control. §P < 0.05 vs. control; vs. §§ control and IL6; † vs. control, IL6, and IL6 + nicotine 10 nM (A, B, D, and E). *P < 0.05 vs. control; #P < 0.05 vs. other group (C). *P < 0.05 or **P < 0.01 vs. IL6, #P < 0.05 vs. other group (F). All two-tailed Student’s t-test. Data are mean ± SEM (n = 4–6/treatment group).

    Journal: Cardiovascular Research

    Article Title: E-cigarette exposure augments murine abdominal aortic aneurysm development: role of Chil1

    doi: 10.1093/cvr/cvac173

    Figure Lengend Snippet: Nicotine induces and augments pro-inflammatory cytokines and ROS in human AoSMC and murine RAW264.7 cells. (A) Nicotine increases expression levels of inflammatory genes (Ccl2, Il6 and Tnf) in murine RAW264.7 cells. (B) Nicotine dose-dependently augments inflammatory gene (Ccl2, Tnf) responses to recombinant IL-6 treatment (20 ng/mL) in RAW264.7 cells. (C) Nicotine dose-dependently increases total ROS in RAW264.7 cells (DCFDA Assay, Ex/Em = 485/535 nm), a process reversed by TEMPOL. (D) Nicotine dose-dependently increases expression levels of inflammatory genes (CCL2, IL6 and IL8) in human AoSMCs. (E) Nicotine dose-dependently augments inflammatory gene (CCL2, IL6 and IL8) responses to recombinant IL-6 treatment (20 ng/mL) in human AoSMCs. (F) Nicotine dose-dependently increases ROS response to IL6 (20 ng/mL) in human AoSMCs (Abcam/Rhodamine, Ex/Em = 550/620 nm). (G) Corresponding pictures to (F) of human AoSMCs stained for ROS under green channel using ROS Detection Assay (Abcam/Rhodamine). Gene expression data are qRT–PCR presented as fold change vs. control. §P < 0.05 vs. control; vs. §§ control and IL6; † vs. control, IL6, and IL6 + nicotine 10 nM (A, B, D, and E). *P < 0.05 vs. control; #P < 0.05 vs. other group (C). *P < 0.05 or **P < 0.01 vs. IL6, #P < 0.05 vs. other group (F). All two-tailed Student’s t-test. Data are mean ± SEM (n = 4–6/treatment group).

    Article Snippet: The DCFDA Cellular Reactive Oxygen Species (ROS) Detection Assay Kit (Abcam/ab113851) was used to quantitatively measure ROS in adherent RAW264.7 cells after 24 h of treatment.

    Techniques: Expressing, Recombinant, Staining, Detection Assay, Quantitative RT-PCR, Two Tailed Test